Formaldehyde assay by capacitance versus voltage and impedance measurements using bi-layer bio-recognition membrane
Ben Ali, M; Korpan, Y; Gonchar, M; El'skaya, A; Maaref, MA; Jaffrezic-Renault, N; Martelet, C
| HERO ID | 1578864 |
|---|---|
| In Press | No |
| Year | 2006 |
| Title | Formaldehyde assay by capacitance versus voltage and impedance measurements using bi-layer bio-recognition membrane |
| Authors | Ben Ali, M; Korpan, Y; Gonchar, M; El'skaya, A; Maaref, MA; Jaffrezic-Renault, N; Martelet, C |
| Journal | Biosensors and Bioelectronics |
| Volume | 22 |
| Issue | 5 |
| Page Numbers | 575-581 |
| Abstract | A novel formaldehyde sensitive biosensor based on bacterial formaldehyde dehydrogenase (FDH) as a bio-recognition element has been developed. The bio-recognition membrane had bi-layer architecture and consisted of FDH, cross-linked with albumin, and of the cofactor NAD at a high concentration level (first layer). The second layer was a negatively charged Nafion membrane, which prevented a leakage of negatively charged NAD molecules from the bio-membrane. As transducers, gold electrodes SiO(2)/Si/SiO(2)/Ti/Au and electrolyte-insulator-semiconductor Si/SiO(2) (EIS) structures have been used. Changes in capacitance and impedance properties of the bio-recognition membrane have been used for monitoring formaldehyde concentration in a bulk solution. It has been shown that formaldehyde can be detected within a concentration range from 1 microM to 20mM depending on the type of transduction used, with a detection limit of 1 and 100 microM for gold-based and EIS-based transducers, respectively. |
| Doi | 10.1016/j.bios.2006.01.019 |
| Pmid | 16516460 |
| Wosid | WOS:000242230800001 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |