Non-enzymatic formation of formaldehyde in mouse oocyte freezing mixtures
Karran, G; Legge, M
HERO ID
1578852
Reference Type
Journal Article
Year
1996
Language
English
PMID
| HERO ID | 1578852 |
|---|---|
| In Press | No |
| Year | 1996 |
| Title | Non-enzymatic formation of formaldehyde in mouse oocyte freezing mixtures |
| Authors | Karran, G; Legge, M |
| Journal | Human Reproduction |
| Volume | 11 |
| Issue | 12 |
| Page Numbers | 2681-2686 |
| Abstract | Three cryoprotectant solvents, dimethylsulphoxide, 1,2-propanediol and glycerol, were investigated for a non-enzymatic reaction product, formaldehyde. All three cryoprotectants demonstrated a direct relationship between increasing solvent molarity and increasing formaldehyde concentration which was independent of temperature and protein (bovine serum albumin). Medium composition significantly influenced the formaldehyde concentration with HTF > T6 > M16 = M2. The formaldehyde could be effectively removed by reduced glutathione, cysteine and dithiothreitol with cysteine being the most effective scavenging agent. A reaction mechanism for this scavenging is proposed. The combination of cysteine and cryoprotectant reduced the zona pellucida 'hardening' effect in mouse oocytes. |
| Doi | 10.1093/oxfordjournals.humrep.a019191 |
| Pmid | 9021372 |
| Wosid | WOS:A1996WE39900024 |
| Url | <Go to ISI>://WOS:A1996WE39900024 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | cryoprotectants; formaldehyde; non-enzymatic reactions; oocytes; thiols |