Copper excess triggers phospholipase D activity in wheat roots
Navari-Izzo, F; Cestone, B; Cavallini, A; Natali, L; Giordani, T; Quartacci, MF
| HERO ID | 1463853 |
|---|---|
| In Press | No |
| Year | 2006 |
| Title | Copper excess triggers phospholipase D activity in wheat roots |
| Authors | Navari-Izzo, F; Cestone, B; Cavallini, A; Natali, L; Giordani, T; Quartacci, MF |
| Journal | Phytochemistry |
| Volume | 67 |
| Issue | 12 |
| Page Numbers | 1232-1242 |
| Abstract | Wheat seedlings (Triticum durum Desf.) were incubated in 100muM Cu(2+) for different periods of time ranging from 1min up to 16h. Following metal addition a rapid intake of copper ions into the roots was observed. Cu(2+) induced an accumulation of both phosphatidic acid and phosphatidylbutanol within 1min of incubation, the latter indicating a very rapid induction of phospholipase D (PLD) activity. The highest PLD stimulation was detected after 2h from copper addition and decreased almost to the initial value at increasing times. Cycloheximide treatment of roots lowered phosphatidylbutanol accumulation because of a reduced PLD activity. The expression profile of a T. durum putative PLD-encoding gene showed a peak after 1h of treatment as well, indicating that enhanced gene expression contributed to the increase in PLD activity. In the absence of copper ions, roots treated with the G protein activator mastoparan showed increases in phosphatidic acid and phosphatidylbutanol similar to those detected with the metal. PLD activity was also stimulated by cholera toxin. Two putatively G protein alpha subunit encoding sequences were isolated and no significant differences in transcription activity following Cu(2+) addition were observed. In copper-treated roots an early production of superoxide generated both by total and membrane-bound NADPH oxidase occurred. The G protein inhibitor suramin as well as the PLD antagonist 1-butanol abolished copper-induced superoxide production. |
| Doi | 10.1016/j.phytochem.2006.04.006 |
| Pmid | 16765389 |
| Wosid | WOS:000239286800008 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000239286800008 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Triticum durum; gramineae; durum wheat; copper; lipids; phosphatidic acid; phospholipase D; reactive oxygen species |