Mutagenicity and cyto-toxicity of n-methyl-2-pyrrolidinone and 4-(methylamino)butanoic acid in the salmonella microsome assay
Wells, DA; Thomas, HF; Digenis, GA
HERO ID
1459767
Reference Type
Journal Article
Year
1988
Language
English
PMID
| HERO ID | 1459767 |
|---|---|
| In Press | No |
| Year | 1988 |
| Title | Mutagenicity and cyto-toxicity of n-methyl-2-pyrrolidinone and 4-(methylamino)butanoic acid in the salmonella microsome assay |
| Authors | Wells, DA; Thomas, HF; Digenis, GA |
| Journal | Journal of Applied Toxicology |
| Volume | 8 |
| Issue | 2 |
| Page Numbers | 135-139 |
| Abstract | The industrial solvent N-methyl-2-pyrrolidinone (NMP) and its hydrolysis product, 4-(methylamino)butanoic acid (N-MeGABA), were examined for mutagenicity and cytotoxicity in the Ames Salmonella/microsome assay. In order to detect a broad range of possible mutagenic endpoints, the following strains were used in the assay: base-pair substitution strains TA100, TA102 and TA104; frameshift strains TA97 and TA98; and repair proficient strains TA2638, UTH8413 and UTH8414. In the standard plate incorporation assay, six log-linear doses of each compound were tested; doses ranged from 0.01 to 1000 mumol/plate for NMP, and 0.01 to 316 mumol/plate for N-MeGABA. Neither compound was detectably mutagenic when tested in the presence and absence of metabolic activation by Aroclor-induced rat liver S9. NMP did show significant responses with strains TA102 and TA104 that were less than two-fold over background, but no clear dose-response relationships were evident. A preincubation modification of the assay was also performed, using strains TA98 and TA104. Mutagenic activity was not observed for NMP, while N-MeGABA showed significant responses with TA104 but dose-related mutagenicity was not established. Preincubation testing revealed both NMP and N-MeGABA to be cytotoxic to the test population of Salmonella at the highest treatment doses. |
| Doi | 10.1002/jat.2550080211 |
| Pmid | 3288686 |
| Wosid | WOS:A1988M883400010 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Relationship(s) |
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