Effect of sodium butyrate and zinc sulphate supplementation on recombinant human IFN-beta production by mammalian cell culture
Zuqueli, R; Prieto, C; Etcheverrigaray, M; Kratje, R
| HERO ID | 1458446 |
|---|---|
| In Press | No |
| Year | 2006 |
| Title | Effect of sodium butyrate and zinc sulphate supplementation on recombinant human IFN-beta production by mammalian cell culture |
| Authors | Zuqueli, R; Prieto, C; Etcheverrigaray, M; Kratje, R |
| Journal | Latin American Applied Research |
| Volume | 36 |
| Issue | 4 |
| Page Numbers | 321-327 |
| Abstract | Recombinant human interferon-beta 1 (rhIFN-beta 1) glycoprotein is used as a therapeutic agent for a variety of diseases, such as multiple sclerosis and hepatitis. In the present work, different strategies were applied to produce rhIFN-beta 1a in mammalian cell cultures. Transfected population of CHO-K1, CHO dhfr(-), BHK and HEK cells were compared for their ability to produce rhIFN-beta 1a, and clones of the most promising cell line (CHO-K1) were isolated by the limit dilution method. Likewise, different culture conditions were assayed by changing the amounts of fetal calf serum, sodium butyrate and/or ZnSO4, to improve cell productivity. The presence of each additive increased the rhIFN-beta 1a yield ranging from 2 to 8 times, depending on the tested cell clone, but when these components were simultaneously added to the medium, the rhIFN-beta 1a concentration in the supernatants was even greater. |
| Wosid | WOS:000242564900019 |
| Is Certified Translation | No |
| Dupe Override | No |
| Conference Name | 2nd MERCOSUR Congress on Chemical Engineering/4th MERCOSUR Congress on Process Systems Engineering (EMPROMER 2005) |
| Conference Date | AUG 14-18, 2005 |
| Comments | Source: Web of Science WOS:000242564900019 |
| Is Public | Yes |
| Language Text | English |
| Keyword | rhIFN-beta 1 a production; CHO cells; zinc sulphate; sodium butyrate |