The interaction of butyrate with TNF-alpha during differentiation and apoptosis of colon epithelial cells: Role of NF-kappa B activation
Hyzd'alova, M; Hofmanova, J; Pachernik, J; Vaculova, A; Kozubik, A
| HERO ID | 1458166 |
|---|---|
| In Press | No |
| Year | 2008 |
| Title | The interaction of butyrate with TNF-alpha during differentiation and apoptosis of colon epithelial cells: Role of NF-kappa B activation |
| Authors | Hyzd'alova, M; Hofmanova, J; Pachernik, J; Vaculova, A; Kozubik, A |
| Journal | Cytokine |
| Volume | 44 |
| Issue | 1 |
| Page Numbers | 33-43 |
| Abstract | We demonstrated that TNF-alpha suppressed differentiation and potentiated cell death induced by butyrate (NaBt) in both adenocarcinoma HT-29 and fetal FHC human colon cells in vitro. Since TNF-alpha is a typical activator of NF-kappaB pathway, we studied the role of NF-kappaB activation in cell differentiation and death during the TNF-alpha and NaBt co-treatment. TNF-alpha induced rapid NF-kappaB activation in both HT-29 and FHC cell lines and this effect was differently modulated by NaBt in these two cell lines. In HT-29 cells, NaBt potentiated NF-kappaB activity induced by TNF-alpha after 4h treatment. However, this initial potentiation of NF-kappaB activity was not observed in FHC cells. During additional time of TNF-alpha and NaBt co-treatment, NaBt decreased the TNF-alpha-mediated NF-kappaB activity in both cell types. We also detected a different response of HT-29 and FHC cells after the pre-treatment with the NF-kappaB inhibitor parthenolide. Our results indicated that NaBt-mediated differentiation and apoptosis of colon epithelial cells can be modulated by TNF-alpha. Furthermore, we found significant differences in the mechanism of the NaBt and TNF-alpha co-treatment effects between cells of non-cancer and cancer origin, suggesting that the NF-kappaB pathway may be more effectively involved in these processes in cancer cells. |
| Doi | 10.1016/j.cyto.2008.06.003 |
| Pmid | 18662886 |
| Wosid | WOS:000260700600007 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000260700600007 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Butyrate; TNF-alpha; Differentiation; Apoptosis; NF-kappa B |