Effects of butyrate on intestinal barrier function in a Caco-2 cell monolayer model of intestinal barrier
Peng, L; He, Z; Chen, Wei; Holzman, IanR; Lin, J
HERO ID
1457527
Reference Type
Journal Article
Year
2007
Language
English
PMID
| HERO ID | 1457527 |
|---|---|
| In Press | No |
| Year | 2007 |
| Title | Effects of butyrate on intestinal barrier function in a Caco-2 cell monolayer model of intestinal barrier |
| Authors | Peng, L; He, Z; Chen, Wei; Holzman, IanR; Lin, J |
| Journal | Pediatric Research |
| Volume | 61 |
| Issue | 1 |
| Page Numbers | 37-41 |
| Abstract | Production of short-chain fatty acids (SCFA) in the intestinal lumen may play an important role in the maintenance of the intestinal barrier. However, overproduction/accumulation of SCFA in the bowel may be toxic to the intestinal mucosa and has been hypothesized to play a role in the pathogenesis of neonatal necrotizing enterocolitis (NEC). By using a Caco-2 cell monolayer model of intestinal barrier, we report here that the effect of butyrate on the intestinal barrier is paradoxical. Butyrate at a low concentration (2 mM) promotes intestinal barrier function as measured by a significant increase in transepithelial electrical resistance (TER) and a significant decrease in inulin permeability. Butyrate at a high concentration (8 mM) reduces TER and increases inulin permeability significantly. Butyrate induces apoptosis and reduces the number of viable Caco-2 cells in a dose-dependent manner. Intestinal barrier function impairment induced by high concentrations of butyrate is most likely related to butyrate-induced cytotoxicity due to apoptosis. We conclude that the effect of butyrate on the intestinal barrier is paradoxical; i.e. whereas low concentrations of butyrate may be beneficial in promoting intestinal barrier function, excessive butyrate may induce severe intestinal epithelial cell apoptosis and disrupt intestinal barrier. |
| Doi | 10.1203/01.pdr.0000250014.92242.f3 |
| Pmid | 17211138 |
| Wosid | WOS:000243045700008 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000243045700008 |
| Is Public | Yes |
| Language Text | English |