Dietary calcium phosphate content and oat beta-glucan influence gastrointestinal microbiota, butyrate-producing bacteria and butyrate fermentation in weaned pigs
Metzler-Zebeli, BU; Zijlstra, RT; Mosenthin, R; Gaenzle, MG
HERO ID
1457384
Reference Type
Journal Article
Year
2011
Language
English
PMID
| HERO ID | 1457384 |
|---|---|
| In Press | No |
| Year | 2011 |
| Title | Dietary calcium phosphate content and oat beta-glucan influence gastrointestinal microbiota, butyrate-producing bacteria and butyrate fermentation in weaned pigs |
| Authors | Metzler-Zebeli, BU; Zijlstra, RT; Mosenthin, R; Gaenzle, MG |
| Journal | FEMS Microbiology Ecology |
| Volume | 75 |
| Issue | 3 |
| Page Numbers | 402-413 |
| Abstract | This study aimed to evaluate the effects of oat β-glucan in combination with low- and high-dietary calcium phosphate (CaP) content on gastrointestinal bacterial microbiota, prevalence of butyrate-production pathway genes and fermentation end-products in 32 weaned pigs allocated to four diets: a cornstarch-casein-based diet with low [65% of the calcium (Ca) and phosphorous (P) requirement] and high CaP content (125% and 115% of the Ca and P requirement, respectively); and low and high CaP diets supplemented with 8.95% of oat β-glucan concentrate. Pigs were slaughtered after 14 days, and digesta were collected for quantitative PCR analysis, and quantification of short-chain fatty acids and lactate. The high CaP content reduced gastric lactate and streptococci and propionate in the large intestine. Oat β-glucan distinctly raised gastric bacterial numbers, and colonic lactobacilli and bifidobacteria. Although not reflected by gene copies of butyrate-production pathway genes, oat β-glucan also increased gastric, caecal and colonic butyrate concentrations, which may be favourable for intestinal development in weaned pigs. Thus, a high CaP content negatively affected the intestinal abundance of certain fermentation end-products, whereas oat β-glucan generally enhanced bacterial numbers and activity. The results emphasize the importance of the stomach for bacterial metabolism of oat β-glucan in weaned pigs. |
| Doi | 10.1111/j.1574-6941.2010.01017.x |
| Pmid | 21166688 |
| Wosid | WOS:000286513000005 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000286513000005 |
| Is Public | Yes |
| Language Text | English |
| Keyword | bacterial community; butyrate; calcium phosphate; oat beta-glucan; quantitative PCR; weaned pigs |