Immunosuppressive properties of Pluchea lanceolata leaves
Bhagwat, DP; Kharya, MD; Bani, S; Kaul, A; Kour, K; Chauhan, PS; Suri, KA; Satti, NK
| HERO ID | 1455300 |
|---|---|
| In Press | No |
| Year | 2010 |
| Title | Immunosuppressive properties of Pluchea lanceolata leaves |
| Authors | Bhagwat, DP; Kharya, MD; Bani, S; Kaul, A; Kour, K; Chauhan, PS; Suri, KA; Satti, NK |
| Journal | Indian Journal of Pharmacology |
| Volume | 42 |
| Issue | 1 |
| Page Numbers | 21-26 |
| Abstract | <strong>OBJECTIVE: </strong>To investigate the immunosuppressive potential of Pluchea lanceolata 50% ethanolic extract (PL) and its bioactive chloroform fraction (PLC).<br /><br /><strong>MATERIALS AND METHODS: </strong>Preliminary screening of the Pluchea lanceolata 50% ethanolic extract (PL) was carried out with basic models of immunomodulation, such as, the humoral antibody response (hemagglutination antibody titers), cell-mediated immune response (delayed-type hypersensitivity), skin allograft rejection test, in vitro (C. albicans method), and in vivo phagocytosis (carbon clearance test). The extract was then fractionated with chloroform, n-butanol, and water to receive the respective fractions by partitioning. These fractions were employed for flow cytometry to study the T-cell specific immunosuppressive potential of these fractions.<br /><br /><strong>RESULTS: </strong>Oral administration of PL at doses of 50 to 800 mg/kg in mice, with sheep red blood cells (SRBC) as an antigen, inhibited both humoral and cell-mediated immune responses, as evidenced by the production of the circulating antibody titer and delayed-type hypersensitiviy reaction results, respectively, and the immune suppression was statistically significant (P < 0.01) in Balb/C mice. PL also decreased the process of phagocytosis both in vitro (31.23%) and ex vivo (32.81%) and delayed the graft rejection time (30.76%). To study the T-cell-specific activities, chloroform, n-butanol, and water fractions from P. lanceolata were tested for T-cell specific immunosuppressive evaluation, wherein only the chloroform fraction (PLC) showed significant (P < 0.01) suppression of CD8+ / CD4+ T-cell surface markers and intracellular Th1 (IL-2 and IFN-(Y)) cytokines at 25 - 200 mg/kg p.o. doses. PLC, however, did not show significant suppression of the Th2 (IL-4) cytokine.<br /><br /><strong>CONCLUSION: </strong>The findings from the present investigation reveal that P. lanceolata causes immunosuppression by inhibiting Th1 cytokines. |
| Doi | 10.4103/0253-7613.62405 |
| Pmid | 20606832 |
| Wosid | WOS:000284053000006 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000284053000006 |
| Is Public | Yes |
| Language Text | English |
| Keyword | CD 4 T-helper cells; CD 8 T-cytotoxic cells; cell-mediated immune response; Humoral antibody titer; Pluchea lanceolata |