Co-expression of an organic solvent-tolerant lipase and its cognate foldase of Pseudomonas aeruginosa CS-2 and the application of the immobilized recombinant lipase
Peng, R; Lin, J; Wei, D
| HERO ID | 1455163 |
|---|---|
| In Press | No |
| Year | 2011 |
| Title | Co-expression of an organic solvent-tolerant lipase and its cognate foldase of Pseudomonas aeruginosa CS-2 and the application of the immobilized recombinant lipase |
| Authors | Peng, R; Lin, J; Wei, D |
| Journal | Applied Biochemistry and Biotechnology |
| Volume | 165 |
| Issue | 3-4 |
| Page Numbers | 926-937 |
| Abstract | The genes of CS-2 lipase and its cognate foldase were cloned from Pseudomonas aeruginosa CS-2. A stop codon was not found in the lipase gene. The amino acid sequence deduced from the lipase gene from P. aeruginosa CS-2 showed 97.8%, 71.3%, and 71.2% identity with lipases from P. aeruginosa LST-03, P seudomonas mendocina ymp, and Pseudomonas stutzeri A1501, respectively. The co-expression of CS-2 lipase and its cognate foldase of P. aeruginosa CS-2 in E scherichia coli BL21 (DE3) resulted in the formation of a soluble lipase. The recombinant lipase and foldase were purified to homogeneity using nickel affinity chromatography and about 10.2-fold with 40.9% recovery was achieved for the purification of the recombinant lipase. The molecular masses of the lipase and the foldase were estimated to be 35.7 and 38.3 kDa in SDS-PAGE, respectively. The recombinant lipase showed stability in the presence of some organic solvents. The recombinant CS-2 lipase was immobilized and subsequently used for the synthesis of butyl acetate in heptane. The conversion of substrate decreased from 98.2% to 87.4% after 5 cycles in reuse of the immobilized lipase. |
| Doi | 10.1007/s12010-011-9309-9 |
| Pmid | 21720839 |
| Wosid | WOS:000296640000016 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000296640000016 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Lipase; Foldase; Pseudomonas aeruginosa; Butyl acetate |