Oscillibacter ruminantium sp. nov., isolated from the rumen of Korean native cattle
Lee, GH; Rhee, MS; Chang, DH; Lee, J; Kim, S; Yoon, MH; Kim, BC
| HERO ID | 1454617 |
|---|---|
| In Press | No |
| Year | 2012 |
| Title | Oscillibacter ruminantium sp. nov., isolated from the rumen of Korean native cattle |
| Authors | Lee, GH; Rhee, MS; Chang, DH; Lee, J; Kim, S; Yoon, MH; Kim, BC |
| Journal | International Journal of Systematic and Evolutionary Microbiology |
| Abstract | A strictly anaerobic, Gram-negative, motile, non-spore-forming bacterial strain GH1 was isolated from the rumen fluid of Korean native cattle (Hanwoo). Cells were straight to slightly curved rods, 2.0-4.5 um long and were motile by peritrichous flagella. The temperature range for growth was 30-45 °C (optimum 40 °C) and the pH range was between 5.5 and 6.5 (optimum 6.0). Growth occurred up to 3.5% (w/v) NaCl. Strain GH1 produced acid from D-glucose and a few pentoses such as D-ribose and D-xylose. Butyric acid was the major end product from these monosaccharides. The genomic DNA G+C content was 54.6 mol%. Based on comparative 16S rRNA gene sequence analysis, strain GH1 belonged phylogenetically to the clostridial cluster IV of the low-G+C-content Gram-positive bacteria and showed the highest 16S rRNA gene sequence similarity to Oscillibacter valericigenes DSM 18026T (97.3 % similarity). DNA-DNA hybridization of strain GH1 and Oscillibacter valericigenes DSM 18026T showed a relatedness value of 24%. The major fatty acids were iso-C13 : 0 (13.0 %), iso-C15 : 0 (17.6 %), anteiso-C15 : 0 (8.4 %), C14 : 0 (4.1 %), and the cellular fatty acid methyl esters as dimethylacetals (DMAs) were DMA16 : 0 (17.8 %), iso-DMA15 : 0 (15.2 %) and DMA14 : 0 (4.52 %). The next phylogenetically close neighbours of strain GH1 were Oscillospira guilliermondii OSC5 (91.6%), Pseudoflavonifractor capillosus ATCC 29799T (91.2%), and Flavonifractor plautii CCUG 28093T (91.2%). Based on 16S rRNA gene sequence similarity, phylogenetic analysis, DNA G+C content, DNA-DNA hybridization data and distinct phenotypic characteristics, strain GH1T (=KCTC 15176T =NBRC 108824T ) was classified in the genus Oscillibacter, as a member of a novel species, for which the name Oscillibacter ruminantium sp. nov. is proposed. |
| Doi | 10.1099/ijs.0.041749-0 |
| Pmid | 23024142 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |