Enhancement of butanol tolerance and butanol yield in Clostridium acetobutylicum mutant NT642 obtained by nitrogen ion beam implantation
Liu, XB; Gu, QY; Yu, XB; Luo, W
HERO ID
1454364
Reference Type
Journal Article
Year
2012
Language
English
PMID
| HERO ID | 1454364 |
|---|---|
| In Press | No |
| Year | 2012 |
| Title | Enhancement of butanol tolerance and butanol yield in Clostridium acetobutylicum mutant NT642 obtained by nitrogen ion beam implantation |
| Authors | Liu, XB; Gu, QY; Yu, XB; Luo, W |
| Journal | Journal of Microbiology |
| Volume | 50 |
| Issue | 6 |
| Page Numbers | 1024-1028 |
| Abstract | As a promising alternative biofuel, biobutanol can be produced through acetone/butanol/ethanol (ABE) fermentation. Currently, ABE fermentation is still a small-scale industry due to its low production and high input cost. Moreover, butanol toxicity to the Clostridium fermentation host limits the accumulation of butanol in the fermentation broth. The wild-type Clostridium acetobutylicum D64 can only produce about 13 g butanol/L and tolerates less than 2% (v/v) butanol. To improve the tolerance of C. acetobutylicum D64 for enhancing the production of butanol, nitrogen ion beam implantation was employed and finally five mutants with enhanced butanol tolerance were obtained. Among these, the most butanol tolerant mutant C. acetobutylicum NT642 can tolerate above 3% (v/v) butanol while the wide-type strain can only withstand 2% (v/v). In batch fermentation, the production of butanol and ABE yield of C. acetobutylicum NT642 was 15.4 g/L and 22.3 g/L, respectively, which were both higher than those of its parental strain and the other mutants using corn or cassava as substrate. Enhancing butanol tolerance is a great precondition for obtaining a hyper-yield producer. Nitrogen ion beam implantation could be a promising biotechnology to improve butanol tolerance and production of the host strain C. acetobutylicum. |
| Doi | 10.1007/s12275-012-2289-9 |
| Pmid | 23274990 |
| Wosid | WOS:000312879300018 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000312879300018 |
| Is Public | Yes |
| Language Text | English |
| Keyword | butanol tolerance; butanol yield; Clostridium acetobutylicum; nitrogen ion beam implantation; fermentation |