Combined utilization of lipase-displaying Pichia pastoris whole-cell biocatalysts to improve biodiesel production in co-solvent media
Jin, Z; Han, SY; Zhang, L; Zheng, SP; Wang, Y; Lin, Y
| HERO ID | 1454326 |
|---|---|
| In Press | No |
| Year | 2013 |
| Title | Combined utilization of lipase-displaying Pichia pastoris whole-cell biocatalysts to improve biodiesel production in co-solvent media |
| Authors | Jin, Z; Han, SY; Zhang, L; Zheng, SP; Wang, Y; Lin, Y |
| Journal | Bioresource Technology |
| Volume | 130 |
| Page Numbers | 102-109 |
| Abstract | Lipase-displaying whole cells appear to be efficient biocatalysts because of their low preparation costs and simple recycling procedure. The combined utilization of Candida antarctica lipase B (CALB) and Rhizomucor miehei lipase (RML), separately displayed on Pichia pastoris whole cells, to produce biodiesel in co-solvent media was investigated. A response surface methodology incorporating a D-optimal design was employed to obtain the optimum reaction conditions for methyl ester (ME) synthesis. The synergistic effect of the two displayed lipases and the use of tert-butanol and isooctane as the co-solvent media were found to significantly improve the transesterification reaction. Scaled-up reactions using various types of feedstock were carried out in a 0.5-l stirred reactor under optimum conditions, affording ME yields over 90% in 12h. Moreover, the ME yields remained above 85% after 20 repeated batch cycles. In conclusion, this biocatalyst affords a promising route to efficient biodiesel production. |
| Doi | 10.1016/j.biortech.2012.12.020 |
| Pmid | 23306117 |
| Wosid | WOS:000316032300015 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Lipase-displaying whole-cell biocatalyst; Biodiesel; Combined utilization of lipases; Co-solvent; Response surface methodology |