Enantioseparation of (R,S)-ketoprofen using Candida antarctica lipase B in an enzymatic membrane reactor
Ong, AL; Kamaruddin, AH; Bhatia, S; Aboul-Enein, HY
HERO ID
1454150
Reference Type
Journal Article
Year
2008
Language
English
PMID
| HERO ID | 1454150 |
|---|---|
| In Press | No |
| Year | 2008 |
| Title | Enantioseparation of (R,S)-ketoprofen using Candida antarctica lipase B in an enzymatic membrane reactor |
| Authors | Ong, AL; Kamaruddin, AH; Bhatia, S; Aboul-Enein, HY |
| Journal | Journal of Separation Science |
| Volume | 31 |
| Issue | 13 |
| Page Numbers | 2476-2485 |
| Abstract | An enzymatic membrane reactor (EMR) for enantioseparation of (R,S)-ketoprofen via Candida antarctica lipase B (CALB) as biocatalyst was investigated. A comparative study of free and immobilized CALB was further conducted. The catalytic behaviour of CALB in an EMR was affected by the process parameters of enzyme load, substrate concentration, substrate molar ratio, lipase solution pH, reaction temperature, and substrate flow rate. Immobilization of CALB in the EMR was able to reduce the amount of enzyme required for the enantioseparation of (R,S)-ketoprofen. Immobilized CALB in the EMR assured higher reaction capacity, better thermal stability, and reusability. It was also found to be more cost effective and practical than free CALB in a batch reactor. |
| Doi | 10.1002/jssc.200800086 |
| Pmid | 18646277 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |