Quantification of chlorpheniramine maleate enantiomers by ultraviolet spectroscopy and chemometric methods
Valderrama, P; Romero, AL; Imamura, PM; Magalhães, IR; Bonato, PS; Poppi, RJ
HERO ID
1454086
Reference Type
Journal Article
Year
2010
Language
English
PMID
| HERO ID | 1454086 |
|---|---|
| In Press | No |
| Year | 2010 |
| Title | Quantification of chlorpheniramine maleate enantiomers by ultraviolet spectroscopy and chemometric methods |
| Authors | Valderrama, P; Romero, AL; Imamura, PM; Magalhães, IR; Bonato, PS; Poppi, RJ |
| Journal | Analytical and Bioanalytical Chemistry |
| Volume | 397 |
| Issue | 1 |
| Page Numbers | 181-188 |
| Abstract | Chlorpheniramine maleate (CLOR) enantiomers were quantified by ultraviolet spectroscopy and partial least squares regression. The CLOR enantiomers were prepared as inclusion complexes with beta-cyclodextrin and 1-butanol with mole fractions in the range from 50 to 100%. For the multivariate calibration the outliers were detected and excluded and variable selection was performed by interval partial least squares and a genetic algorithm. Figures of merit showed results for accuracy of 3.63 and 2.83% (S)-CLOR for root mean square errors of calibration and prediction, respectively. The ellipse confidence region included the point for the intercept and the slope of 1 and 0, respectively. Precision and analytical sensitivity were 0.57 and 0.50% (S)-CLOR, respectively. The sensitivity, selectivity, adjustment, and signal-to-noise ratio were also determined. The model was validated by a paired t test with the results obtained by high-performance liquid chromatography proposed by the European pharmacopoeia and circular dichroism spectroscopy. The results showed there was no significant difference between the methods at the 95% confidence level, indicating that the proposed method can be used as an alternative to standard procedures for chiral analysis. |
| Doi | 10.1007/s00216-010-3472-7 |
| Pmid | 20148242 |
| Wosid | WOS:000276738800021 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000276738800021 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Chlorpheniramine maleate; Enantiomers; Ultraviolet spectroscopy; Multivariate calibration; Validation |