Acute effects of polychlorinated biphenyl-containing and -free transformer fluids on rat testicular steroidogenesis
Andric, SA; Kostic, TS; Dragisic, SM; Andric, NL; Stojilkovic, SS; Kovacevic, RZ
HERO ID
1415639
Reference Type
Journal Article
Year
2000
Language
English
PMID
| HERO ID | 1415639 |
|---|---|
| In Press | No |
| Year | 2000 |
| Title | Acute effects of polychlorinated biphenyl-containing and -free transformer fluids on rat testicular steroidogenesis |
| Authors | Andric, SA; Kostic, TS; Dragisic, SM; Andric, NL; Stojilkovic, SS; Kovacevic, RZ |
| Journal | Environmental Health Perspectives |
| Volume | 108 |
| Issue | 10 |
| Page Numbers | 955-959 |
| Abstract | Polychlorinated biphenyl (PCB)-based transformer fluids belong to a class of environmentally persistent mixtures with known toxic effects. Here, we studied the acute effects of Askarel (which contains Aroclor 1260) and two substitute transformer fluids (the silicone oil-based DC561 and the mineral oil-based ENOL C) on rat testicular steroidogenesis. Single intraperitoneal (ip; 10 mg/kg body weight) or bilateral intratesticular (itt; 25 microg/testis) injections of Askarel markedly decreased serum androgen levels 24 hr after administration. In acute testicular cultures from these animals, chorionic gonadotropin-stimulated progesterone and androgen productions were severely attenuated. When itt was injected or added in vitro, Askarel inhibited 3ss-hydroxysteroid dehydrogenase (3ssHSD), stimulated 17[alpha]-hydroxylase/lyase (P450c17), and did not affect 17ss-hydroxysteroid dehydrogenase in testicular postmitochondrial fractions. The ip-injected Askarel did not affect 3ssHSD, but inhibited P450c17, suggesting that a more intensive metabolism of peripherally injected Askarel reduces the circulating levels of active ingredients below the threshold needed for inhibition of 3ssHSD and generates a derivative that inhibits P450c17. In contrast to Askarel, itt-injection (25 microg/testis) of DC561 and ENOL C did not affect in vivo and in vitro steroidogenesis. These findings show the acute effects of Askarel, but not silicone and mineral oils, on testicular steroidogenesis. |
| Doi | 10.1289/ehp.00108955 |
| Pmid | 11049815 |
| Wosid | WOS:000090093000022 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | 3 beta-hydroxysteroid dehydrogenase; androgen; P450c17; polychlorinated biphenyls; progesterone |
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