Studies on the contamination of blood and plasma proteins by phthalate esters
Vessman, J; Rietz, G
| HERO ID | 1334022 |
|---|---|
| In Press | No |
| Year | 1974 |
| Title | Studies on the contamination of blood and plasma proteins by phthalate esters |
| Authors | Vessman, J; Rietz, G |
| Journal | Developments in Biological Standardization |
| Volume | 27 |
| Page Numbers | 205-208 |
| Abstract | The distribution of di(ethylhexyl)phthalate (117817) (DEHP) in plasma and plasma proteins after fractionation was examined in blood contaminated by storage in plastic. The DEHP content of freshly drawn human plasma, of plasma samples stored in polyethylene containers which did not contain phthalate plasticizers, and of plasma stored over 7 days at 4 degrees-C and at -20 degrees-C was measures. The effects of thawing and refreezing a contaminated sample on DEHP content were studied. Plasma samples were dried after the addition of methanol. The residue, with added internal standard, was suspended in methanol and mixed with toluene. Water was added, the mixture was centrifuged, and the aqueous phase was removed. The toluene layer was analyzed by electron capture gas chromatography for DEHP. The limit of detection was 0.5 micrograms (microg) DEHP per milliliter (ml) of plasma. The relative standard deviation was about 3.7 percent. The DEHP content of freshly drawn plasma did not differ from that of a blank. The DEHP concentration in plasma stored in plastic bags varied between 16 and 120microg/ml. Samples stored in polyethylene containers had high concentrations of DEHP. The DEHP content of plasma increased over 7 days from near zero to 70microg/ml at 4 degrees-C, and from 4 to 26microg/ml at -20 degrees-C. No increase was noted in the thawed and refrozen sample. After fractionation, the major part of DEHP was found in the lipoprotein fraction. Contamination with DEHP ranged from 3 to 18microg/ml in fibrinogen, from 5 to 25microg/ml in albumin, and from 3 to 160microg/ml in immunoglobulin-G. The authors conclude that DEHP is present in very minute amounts (if at all) in human plasma, and that contamination from plastic bags concentrates in the lipoprotein fraction. |
| Pmid | 4463124 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Journal: Developments in biological standardization ISSN: 0301-5149 |
| Is Public | Yes |
| Language Text | English |
| Keyword | <?xml version="1.0" encoding="UTF-8"?><kw>DCN-119972</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Blood analysis</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Phthalates</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Chemical properties</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Sampling methods</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Analytical chemistry</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Biological effects</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Analytical methods</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Chemical analysis</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Laboratory testing</kw> |
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