HIGHLY SENSITIVE AND SPECIFIC DETERMINATION OF PRAVASTATIN SODIUM IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH LASER-INDUCED FLUORESCENCE DETECTION AFTER IMMOBILIZED ANTIBODY EXTRACTION
Dumousseaux, C; Muramatsu, S; Takasaki, W; Takahagi, H
| HERO ID | 1198689 |
|---|---|
| In Press | No |
| Year | 1994 |
| Title | HIGHLY SENSITIVE AND SPECIFIC DETERMINATION OF PRAVASTATIN SODIUM IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH LASER-INDUCED FLUORESCENCE DETECTION AFTER IMMOBILIZED ANTIBODY EXTRACTION |
| Authors | Dumousseaux, C; Muramatsu, S; Takasaki, W; Takahagi, H |
| Journal | Journal of Pharmaceutical Sciences |
| Volume | 83 |
| Issue | 11 |
| Page Numbers | 1630-1636 |
| Abstract | A method for the determination of pravastatin sodium (PS), a cholesterol-lowering agent, in plasma was developed by using an immobilized antibody column extraction followed by high-performance liquid chromatography (HPLC). The analyte was monitored by a laser-induced fluorescence detector after fluorogenic derivatization. The PS antibody was coupled to Sepharose 4B and used as an extraction phase for sample cleanup and extraction of the drug. A plasma sample was applied to the column and washed with water, and the drug was eluted with methanol. N-Dansylethylenediamine (DNS-ED) was coupled to the carboxyl moiety of the drug in the presence of diethyl phosphorocyanidate (DEPC) and triethylamine (TEA) in dioxane. Derivatization was completed in 5 min at room temperature. A column-switching technique was utilized to remove excess reagents and byproducts. A He-Cd laser-induced fluorescence detector was applied to achieve an ultrasensitive determination. The detection limit was 2 pg/injection of PS, which was 20 times more sensitive than the conventional fluorescence detection. The limit of quantitation was 100 pg/mL when 1 mL of plasma sample was available. An average coefficient of variations of the overall method were less than 8% at the concentration range of 1-100 ng/mL. A single oral dose of PS in rats (20 mg/kg) and dogs (5 mg/kg) resulted in average maximum concentrations of 142 sand 310 ng/mL, respectively.< |
| Doi | 10.1002/jps.2600831120 |
| Pmid | 7891286 |
| Wosid | WOS:A1994PQ66100019 |
| Url | https://linkinghub.elsevier.com/retrieve/pii/S0022354915496315 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science A1994PQ66100019 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Antibodies; Indicators and Reagents; Platelet Activating Factor; Pravastatin; KXO2KT9N0G; Index Medicus; Enzyme-Linked Immunosorbent Assay; Rats, Wistar; Spectrometry, Fluorescence; Chromatography, High Pressure Liquid; Animals; Antibodies -- isolation & purification; Platelet Activating Factor -- immunology; Antibodies -- analysis; Platelet Activating Factor -- isolation & purification; Pravastatin -- immunology; Pravastatin -- blood; Pravastatin -- pharmacokinetics |
| Is Qa | No |