Determination of glutathione transferase (GSTT1-1) activities in different tissues based on formation of radioactive metabolites using 35S-glutathione
Thier, R; Delbanco, EH; Wiebel, FA; Hallier, E; Bolt, HM
HERO ID
11962
Reference Type
Journal Article
Year
1998
Language
English
PMID
| HERO ID | 11962 |
|---|---|
| In Press | No |
| Year | 1998 |
| Title | Determination of glutathione transferase (GSTT1-1) activities in different tissues based on formation of radioactive metabolites using 35S-glutathione |
| Authors | Thier, R; Delbanco, EH; Wiebel, FA; Hallier, E; Bolt, HM |
| Journal | Archives of Toxicology |
| Volume | 72 |
| Issue | 12 |
| Page Numbers | 811-815 |
| Abstract | A new system has been developed to determine enzyme activities of glutathione transferase theta (GSTT1-1) based on radiometric product detection resulting from the enzymic reaction of methyl chloride with 35S-labelled glutathione. In principle, the method is universally applicable for determination-of glutathione transferase activities towards a multiplicity of substrates. The method distinguishes between erythrocyte GSTT1-1 activities of human 'non-conjugators', 'low conjugators' and 'high conjugators'. Application to cytosol preparations of livers and kidneys of male and female Fischer 344 and B6C3F1 mice reveals differential GSTT1-1 activities in hepatic and renal tissues. These ought to be considered in species-specific modellings of organ toxicities of chlorinated hydrocarbons. |
| Doi | 10.1007/s002040050578 |
| Pmid | 9950079 |
| Wosid | WOS:000078265400009 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | ECRIB. 35S: 35 is superscript.Arch. Toxicol. 72: 811-815. |
| Is Public | Yes |
| Language Text | English |
| Keyword | Rats; Glutathione; Animals; Glutathione Transferase; Humans; Erythrocytes; Index Medicus; Substrate Specificity; Rats, Inbred F344; Sulfur Radioisotopes; Liver; Female; Methyl Chloride; Mice; Male; Organ Specificity; Kidney |
| Is Peer Review | No |
| Relationship(s) |
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