SENSITIVE ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR THE MYCOTOXIN ZEARALENONE IN BARLEY AND JOBS-TEARS
Tanaka, T; Teshima, R; Ikebuchi, H; Sawada, J; Ichinoe, M
| HERO ID | 1195547 |
|---|---|
| In Press | No |
| Year | 1995 |
| Title | SENSITIVE ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR THE MYCOTOXIN ZEARALENONE IN BARLEY AND JOBS-TEARS |
| Authors | Tanaka, T; Teshima, R; Ikebuchi, H; Sawada, J; Ichinoe, M |
| Journal | Journal of Agricultural and Food Chemistry |
| Volume | 43 |
| Issue | 4 |
| Page Numbers | 946-950 |
| Abstract | An easy, sensitive, competitive indirect enzyme-linked immunosorbent assay (CI-ELISA) for zearalenone in barley and Job's-tears was established. To improve sensitivity of the assay system for zearalenone, a solid phase antigen was prepared by affinity purification. This assay system uses a purified antigen and specific monoclonal antibodies for zearalenone. Consequently, the detection limit of zearalenone by CI-ELISA was increased to 0.2 ng/mL (equivalent to 10 ng/g in barley and Job's-tears). Zearalenone in barley and Job's-tears samples could be determined in the range of 25-1000 ng/g using method A (methanol-water and dichloromethane extraction) and method B (methanol-water alone) as sample preparation. The average recoveries of zearalenone from barley samples were observed to be 106% (mean CV, 10.3%) by method A and 98% (mean CV, 7.5%) by method B; also, those of zearalenone from Job's-tears samples were observed to be 96% (mean CV, 9.4%) by method A and 102% (mean CV, 6.5%) by method B. There was little or no difference between method A and method B for the recovery. For the benefit of a facile sample preparation, unknown amounts of zearalenone in barley and Job's-tears samples were assayed by method B. The results obtained by CI-ELISA were closely correlated with those of high-performance Liquid chromatography. |
| Wosid | WOS:A1995QU38100019 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science A1995QU38100019 |
| Is Public | Yes |
| Keyword | ENZYME-LINKED IMMUNOSORBENT ASSAY; PURIFIED ANTIGEN; ZEARALENONE; BARLEY; JOBS TEARS |
| Is Qa | No |