Application of micronucleus in vitro assay to micropollutants in river water
Chung, YS; Ichikawa, K; Utsumi, H
| HERO ID | 1186821 |
|---|---|
| In Press | No |
| Year | 1997 |
| Title | Application of micronucleus in vitro assay to micropollutants in river water |
| Authors | Chung, YS; Ichikawa, K; Utsumi, H |
| Journal | Water Science and Technology |
| Volume | 35 |
| Issue | 8 |
| Page Numbers | 9-13 |
| Abstract | To determine the genotoxicity of river water towards mammalian cells, we applied Micronucleus in vitro test using mammalian cells to the samples taken from river Tamagawa located between Tokyo and Kanagawa prefecture. Water samples were condensed by Sep-pak cartridges and extracted by dichloromethane and methanol. Positive genotoxicity was observed in methanol extracts from sampling stations of Hinobashi and Marukobashi, while no dichloromethane extracts showed genotoxicity, suggesting that polar genotoxic micropollutants may be contained in the water of Tamagawa, at least in its down-stream. Significantly high mutagenicity also detected from Hinobashi and Marukobashi by Ames mutagenicity test using Salmonella Typhimurium, and some difference was obtained in sensitivity between the two methods. This may arise from the difference in species used, that is, mammalian cells in micronucleus test and bacteria in Ames mutagenicity test. (C) 1997 IAWQ. Published by Elsevier Science Ltd. |
| Wosid | WOS:A1997XK23500003 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science A1997XK23500003 |
| Is Public | Yes |
| Keyword | genotoxicity; micronucleus in vitro test; Ames Salmonella test; mammalian cell line; water safety |
| Is Qa | No |