Analysis of free and esterified ergosterol in tomato products
de Sio, F; Laratta, B; Giovane, A; Quagliuolo, L; Castaldo, D; Servillo, L
| HERO ID | 1174950 |
|---|---|
| In Press | No |
| Year | 2000 |
| Title | Analysis of free and esterified ergosterol in tomato products |
| Authors | de Sio, F; Laratta, B; Giovane, A; Quagliuolo, L; Castaldo, D; Servillo, L |
| Journal | Journal of Agricultural and Food Chemistry |
| Volume | 48 |
| Issue | 3 |
| Page Numbers | 780-784 |
| Abstract | A new extraction and chromatographic procedure to quantify free and esterified ergosterol in tomato products was devised. The extraction solution was composed of a dichloromethane/methanol mixture in a 2:1 (v/v) ratio. This extraction solvent allowed for higher ergosterol recovery from tomato products (an average of 25% more) compared to hexane, which is frequently employed for ergosterol extraction. Both free and esterified ergosterol were determined by HPLC reverse-phase chromatography employing a Nova-Pall C-18 column (300 x 3.9 mm), filled with 4 mm average particle size and a guard column of the same material. The elution was performed at a now rate of I ml min-l with a linear gradient of solvent A (methanol/water, 80:20, v/v) and solvent B (dichloromethane). The gradient, starting at sample injection, was from 0 to 50% B for 20 min for the free ergosterol analysis and additional 15 min at 50% B to analyze the ergosterol esters. This technique has proven to be more sensitive for ergosterol determination than other reported chromatographic procedures. Moreover, ergosterol esters, extracted from various fungal sources, separated well and were easily quantified. |
| Doi | 10.1021/jf990475d |
| Pmid | 10725149 |
| Wosid | WOS:000086026000030 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science 000086026000030Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034107481&doi=10.1021%2fjf990475d&partnerID=40&md5=c814aa5d3a12f3e1ef24708793accdcb |
| Is Public | Yes |
| Language Text | English |
| Keyword | ergosterol; tomato; fungal contamination; microbiological quality |
| Is Qa | No |