Cyclodextrin-modified monolithic columns for resolving dansyl amino acid enantiomers and positional isomers by capillary electrochromatography
Chen, ZL; Ozawa, H; Uchiyama, K; Hobo, T
| HERO ID | 1158244 |
|---|---|
| In Press | No |
| Year | 2003 |
| Title | Cyclodextrin-modified monolithic columns for resolving dansyl amino acid enantiomers and positional isomers by capillary electrochromatography |
| Authors | Chen, ZL; Ozawa, H; Uchiyama, K; Hobo, T |
| Journal | Electrophoresis |
| Volume | 24 |
| Issue | 15 |
| Page Numbers | 2550-2558 |
| Abstract | We describe beta- and gamma-cyclodextrins (beta- and gamma-CD)-modified monolithic columns prepared by sol-gel process and chemical modifications. The monolithic silica column was fabricated inside a fused-silica capillary with 100 microm inner diameter by sol-gel process. The monolithic silica matrix was chemically modified chiral selectors of beta- or gamma-CDs with a spacer of 3-glycidoxypropyltrimethoxysilane by on-column reactions. Gamma-CD-modified monolithic column has successfully been applied for the separation of dansyl amino acid enantiomers. Beta-CD-modified monolithic column has been used for the separation of the positional isomers of o-, m-, and p-cresols and the enantioseparation of racemates of benzoin and several dansyl amino acids by capillary electrochromatography, respectively. For the separation of neutral positional isomers, a positive electric field was applied. However, for the separation of negatively charged analytes, a negative electric field was applied at the inlet of column. The separation efficiency of 5.0 x 10(4) theoretical plates/m for dansyl-L-threonine was obtained at electric field strength of -300 V/cm in the mobile phase of 50 mM 2-(N-morpholino)ethanesulfonic acid (MES)-Tris/methanol (70/30) buffer at pH 7.0. L-enantiomers were eluted as the first peak. Scanning electron micrograph showed that monolithic columns have the morphology of continuous skeleton and large through-pores. |
| Doi | 10.1002/elps.200305403 |
| Pmid | 12900867 |
| Wosid | WOS:000184932400018 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science 000184932400018 |
| Is Public | Yes |
| Language Text | English |
| Keyword | capillary electrochromatography; chiral separation; cyclodextrin; dansyl amino acids; monolithic column; positional isomers; sol-gel process |
| Is Qa | No |