Efficient purification and morphology characterization of paclitaxel from cell cultures of Taxus chinensis
Pyo, SH; Kim, MS; Cho, JS; Song, BK; Han, BH; Choi, HJ
| HERO ID | 1154806 |
|---|---|
| In Press | No |
| Year | 2004 |
| Title | Efficient purification and morphology characterization of paclitaxel from cell cultures of Taxus chinensis |
| Authors | Pyo, SH; Kim, MS; Cho, JS; Song, BK; Han, BH; Choi, HJ |
| Journal | Journal of Chemical Technology and Biotechnology |
| Volume | 79 |
| Issue | 10 |
| Page Numbers | 1162-1168 |
| Abstract | Paclitaxel was purified from cell cultures of Taxus chinensis by a combination of extraction, low-pressure chromatography, precipitation and HPLC. A crude extract (purity 6.9%) was obtained by methanol extraction of plant cell cultures, followed by liquid-liquid extraction using dichloromethane. The extract was purified to greater than 32% with a 97% step yield by low-pressure chromatography. After acetone/pentane precipitation, the resulting purity and step yield were 75.8% and 97.4%, respectively. High performance liquid chromatography steps, which were composed of an HPLC step with column-packed ODS and an HPLC step with column-packed silica, were applied to give over 99.5% purity with high yield. Amorphous paclitaxel with a fine particle size, which has a solubility advantage compared with the stable crystalline form, was obtained by dissolving in dichloromethane, followed by spray-drying. (C) 2004 Society of Chemical Industry. |
| Doi | 10.1002/jctb.1111 |
| Wosid | WOS:000224308700018 |
| Url | http://doi.wiley.com/10.1002/jctb.1111 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science 000224308700018 |
| Is Public | Yes |
| Keyword | paclitaxel; purification; silica low-pressure chromatography; acetone/pentane precipitation; amorphous; spray-drying |
| Is Qa | No |