Combining microwave-assisted extraction and liquid chromatography-ion-trap mass spectrometry for the analysis of hexabromocyclododecane diastereoisomers in marine sediments
Wu, HH; Chen, HC; Ding, WH
HERO ID
1129680
Reference Type
Journal Article
Year
2009
Language
English
PMID
| HERO ID | 1129680 |
|---|---|
| In Press | No |
| Year | 2009 |
| Title | Combining microwave-assisted extraction and liquid chromatography-ion-trap mass spectrometry for the analysis of hexabromocyclododecane diastereoisomers in marine sediments |
| Authors | Wu, HH; Chen, HC; Ding, WH |
| Journal | Journal of Chromatography A |
| Volume | 1216 |
| Issue | 45 |
| Page Numbers | 7755-7760 |
| Abstract | An efficient microwave-assisted extraction (MAE) procedure coupled with high performance liquid chromatography-electrospray-ion-trap mass spectrometry (HPLC-ESI-ITMS) has been evaluated to determine hexabromocyclododecane diastereoisomers (alpha-, beta- and gamma-HBCD) in marine sediments. The composition of the LC mobile phase (consisting of water, methanol and acetonitrile) and the parameters of electrospray ionization (ESI) were evaluated to obtain chromatographic baseline separation and high sensitivity for the detection of these diastereoisomers. The effects of various operating parameters on the quantitative extraction of the HBCDs through MAE were systematically investigated. The three diastereoisomers were then quantitated by HPLC-ITMS employing ESI operated in the negative ionization mode. The HBCDs were extracted from the sediments through MAE using 40mL of acetone/n-hexane (1/3, v/v) at 90 degrees C for 12min. The limits of quantitation (LOQ) ranged from 25 to 40pg/g (dry weight) in 5g of the sediment samples. The recoveries of the HBCDs in spiked sediment samples ranged from 68 to 91% (relative standard derivation: 2-11%). The extraction efficiency of the MAE technique was also compared with Soxhlet extraction and pressurized liquid extraction. |
| Doi | 10.1016/j.chroma.2009.09.001 |
| Pmid | 19765716 |
| Wosid | WOS:000271408000020 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science 000271408000020Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-70349798586&doi=10.1016%2fj.chroma.2009.09.001&partnerID=40&md5=a5956410c911dadd080ec8e08c080df6 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Hexabromocyclododecane; Microwave-assisted extraction; Liquid chromatography; Mass spectrometry; Sediment analysis |
| Is Qa | No |