Flavonoids in Scutellaria immaculata and S. ramosissima (Lamiaceae) and their biological activity
Mamadalieva, NZ; Herrmann, F; El-Readi, MZ; Tahrani, A; Hamoud, R; Egamberdieva, DR; Azimova, SS; Wink, M
| HERO ID | 1040040 |
|---|---|
| In Press | No |
| Year | 2011 |
| Title | Flavonoids in Scutellaria immaculata and S. ramosissima (Lamiaceae) and their biological activity |
| Authors | Mamadalieva, NZ; Herrmann, F; El-Readi, MZ; Tahrani, A; Hamoud, R; Egamberdieva, DR; Azimova, SS; Wink, M |
| Journal | Journal of Pharmacy and Pharmacology |
| Volume | 63 |
| Issue | 10 |
| Page Numbers | 1346-1357 |
| Abstract | <strong>OBJECTIVES: </strong>The aim of this study was to investigate the flavonoid composition of Scutellaria immaculata and S. ramosissima (Lamiaceae) and the in-vitro biological activity of their extracts and flavonoids.<br /><br /><strong>METHODS: </strong>The flavonoid composition of S. immaculata (Si) and S. ramosissima (Sr) were analysed using LC-MS. Antimicrobial activity was studied in vitro against a range of bacteria and fungi using diffusion and microdilution methods. Anti-trypanosomal and cell proliferation inhibitory activity of the extracts and flavonoids was assessed using MTT. The antioxidant activity of the flavonoids and extracts were evaluated using DPPH* test.<br /><br /><strong>KEY FINDINGS: </strong>LC-MS investigation of Si and Sr plants allowed the identification, for the first time, of an additional 9 and 16 flavonoids, respectively. The methanol, chloroform and water extracts from these plants and six flavonoids (scutellarin, chrysin, apigenin, apigenin-7-O-glucoside, cynaroside and pinocembrine) exhibited significant inhibition of cell growth against HeLa, HepG-2 and MCF-7 cells. The chloroform extract of Sr showed potent cytotoxic effects with IC50 (drug concentration which resulted in a 50% reduction in cell viability) values of 9.25 ± 1.07 µg/ml, 12.83 ± 1.49 µg/ml and 17.29 ± 1.27 µg/ml, respectively. The highest anti-trypanosomal effect against T. b. brucei was shown by the chloroform extract of Sr with an IC50 (drug concentration which resulted in a 50% inhibition of the biological activity) of 61 µg/ml. The pure flavonoids showed an IC50 range between 3 and 29 µm, with cynaroside as the most active compound with an IC50 value of 3.961 ± 0.133 µm. The chloroform extract of Sr has potent antimicrobial activity against Streptococcus pyogenes (minimum inhibitory concentration, MIC = 0.03 mg/ml). Pinocembrine exhibited a strong activity against the all bacteria except Escherichia coli and yeasts. Water extracts of Sr and Si exhibited potent antioxidant activity with IC50 values of 5.62 ± 0.51 µg/ml and 3.48 ± 0.02 µg/ml, respectively. Scutellarin exerted stronger antioxidant activity than other flavonoids.<br /><br /><strong>CONCLUSIONS: </strong>This is the first study reporting an in-vitro biological investigation for Si and Sr. Especially the chloroform extract of Sr showed potent anticancer and antimicrobial activity. Cynaroside had a highly selective and strong cytotoxicity against T. b. brucei while showing only mild effects against cancer cells. |
| Doi | 10.1111/j.2042-7158.2011.01336.x |
| Pmid | 21899551 |
| Wosid | WOS:000295093500012 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science 000295093500012 |
| Is Public | Yes |
| Language Text | English |
| Keyword | antimicrobial; anti-trypanosomal; cytotoxicity; Scutellaria immaculata; S. ramosissima |
| Is Qa | No |