In vitro susceptibility of Mycobacterium tuberculosis to extracts of Eucalyptus camaldulensis and Eucalyptus torelliana and isolated compounds
Lawal, TO; Adeniyi, BA; Adegoke, AO; Franzblau, SG; Mahady, GB
| HERO ID | 1039308 |
|---|---|
| In Press | No |
| Year | 2012 |
| Title | In vitro susceptibility of Mycobacterium tuberculosis to extracts of Eucalyptus camaldulensis and Eucalyptus torelliana and isolated compounds |
| Authors | Lawal, TO; Adeniyi, BA; Adegoke, AO; Franzblau, SG; Mahady, GB |
| Journal | Pharmaceutical Biology |
| Volume | 50 |
| Issue | 1 |
| Page Numbers | 92-98 |
| Abstract | <strong>CONTEXT: </strong>Eucalyptus camaldulensis Dehnh. (Myrtaceae) and Eucalyptus torelliana F. Muell are used in Nigerian traditional medicine for the treatment of cough associated with tuberculosis (TB) and other respiratory infections.<br /><br /><strong>OBJECTIVE: </strong>Hexane, chloroform, methanol extracts, and isolated compounds of E. camaldulensis and E. torelliana were screened for activity against Mycobacterium tuberculosis H37Rv (MtbH37Rv) to authenticate the traditional use of these plants.<br /><br /><strong>MATERIALS AND METHODS: </strong>The microplate alamar blue assay (MABA) method was used to investigate the anti-M. tuberculosis activities. Bioassay-guided fractionation of the hexane extract of E. torelliana leaf was performed, and isolated compounds were characterized by MS, 1D- and 2D-NMR.<br /><br /><strong>RESULTS: </strong>The extracts inhibited the growth of MtbH37Rv [minimum inhibitory concentration (MIC) 4-64 µg/mL]. Spectroscopic characterization led to the identification of two compounds, hydroxymyristic acid methylester (1) and a substituted pyrenyl ester, a sterol (2). Compounds 1 and 2 had MIC of 49.45 and 46.99 µg/mL; IC(50) >100 and 38.21 µg/mL; selectivity index (SI) >2.02 and 0.81, respectively, and a minimum bactericidal concentration (MBC) of 62.50 µg/mL.<br /><br /><strong>DISCUSSION AND CONCLUSIONS: </strong>The anti-TB activities of these plants on M. tuberculosis H37Rv support their use in traditional medicine for the treatment of coughs associated with TB and reveals the presence of anti-Mtb active compounds in the plants. These findings not only demonstrate a new potential area of therapeutic value of E. camaldulensis and E. torelliana, but also illustrate the role of esters as anti-Mtb active principles in ethnobotanical preparations and as lead compounds in the development of new and effective anti-Mtb drugs. |
| Doi | 10.3109/13880209.2011.625953 |
| Pmid | 22129202 |
| Wosid | WOS:000298380200011 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science 000298380200011Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84555190263&doi=10.3109%2f13880209.2011.625953&partnerID=40&md5=a1bc6e1787f17d05479bde923a927f50 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Respiratory tract infection; tuberculosis; microplate Alamar blue assay; bioassay-guided fractionation; selectivity index; traditional medicine; ethnobotanical |
| Is Qa | No |