Antiproliferative property of n-hexane and chloroform extracts of Anisomeles malabarica (L). R. Br. in HPV16-positive human cervical cancer cells
Preethy, CP; Padmapriya, R; Periasamy, VS; Riyasdeen, A; Srinag, S; Krishnamurthy, H; Alshatwi, AA; Akbarsha, MA
| HERO ID | 1038049 |
|---|---|
| In Press | No |
| Year | 2012 |
| Title | Antiproliferative property of n-hexane and chloroform extracts of Anisomeles malabarica (L). R. Br. in HPV16-positive human cervical cancer cells |
| Authors | Preethy, CP; Padmapriya, R; Periasamy, VS; Riyasdeen, A; Srinag, S; Krishnamurthy, H; Alshatwi, AA; Akbarsha, MA |
| Journal | Journal of Pharmacology and Pharmacotherapeutics |
| Volume | 3 |
| Issue | 1 |
| Page Numbers | 26-34 |
| Abstract | <strong>OBJECTIVES: </strong>To find the efficacy of serial extracts of Anisomeles malabarica in inhibiting proliferation of and inducing apoptosis in human cervical cancer cells, SiHa and ME 180, that are HPV 16-positive.<br /><br /><strong>MATERIALS AND METHODS: </strong>The whole plant was extracted in n-hexane, chloroform, ethyl acetate, n-butanol, methanol, and water. The cells were treated with the extracts at increasing concentrations to find the IC(50), adopting MTT ([3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]) assay. Acridine orange (AO) and ethidium bromide (EB) and Hoechst 33258 staining were adopted to assess the mode of cell death, Annexin V-Cy3 staining to evaluate one of the early apoptotic features, JC-1 staining to assess the mitochondrial membrane depolarization, comet assay for DNA fragmentation, and cell cycle analysis for the distribution of cells after treatment.<br /><br /><strong>RESULTS: </strong>n-Hexane and chloroform extracts were cytotoxic to the cervical cancer cells in dose- and duration-dependent manner. The cells that responded to the treatments revealed typical apoptotic features. Early features of apoptosis, phosphatidyl serine translocation and loss of mitochondrial trans-membrane potential, were observed in the treated cells, and comet assay revealed DNA damage. In the FACS analysis, the cells accumulated in the sub-G0/G1 phase of the cell cycle, except in n-hexane- and chloroform extract-treated SiHa cells at 24 h, which showed arrest in S- and G2/M phases.<br /><br /><strong>CONCLUSIONS: </strong>n-Hexane and chloroform extracts of A. malabarica inhibit proliferation of and induce death in HPV16-positive cervical cancer cells, mostly by apoptosis and to some extent by necrosis. |
| Doi | 10.4103/0976-500X.92500 |
| Pmid | 22368413 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Journal: Journal of pharmacology & pharmacotherapeutics ISSN: 0976-5018Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84871950336&doi=10.4103%2f0976-500X.92500&partnerID=40&md5=1b1c4546b80857ff822b65002d915ce6 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Anti-cancer; apoptosis; cell cycle arrest; cervical cancer |
| Is Qa | No |