Abstract  Core Ideas: Farmers struggle to maintain and balance economic and environmental sustainability. Identification of knowledge gaps related to crop residue management. Discussion of crop residue manage expanded from the U.S. Midwest to a global perspective. Use of carbon flux tower data to validate simulation models. Crop residue harvesting impacts soil health, productivity, and greenhouse gas emissions. The amount of crop residues that can be sustainability removed is highly variable and is a function of many factors including the soil, climatic, and plant characteristics. For example, leaving an insufficient amount of crop residue on the soil surface can be detrimental for soil quality, result in loss of soil organic matter (SOM), and increase soil erosion, whereas leaving excessive amounts can impair soil-seed contact, immobilize N, and/or keep soils cool and wet. This special issue evolved as an outcome of, “Crop Residues for Advanced Biofuels: Effects on Soil Carbon” workshop held in Sacramento, CA, in 2017. The goal of the special issue is to provide a forum for identifying knowledge gaps associated with crop residue management and to expand the discussion from a regional Midwestern U.S. to a global perspective. Several crop residue experiments as well as simulation modeling studies are included to examine effects of tillage, crop rotation, livestock grazing, and cover crops on greenhouse gas (GHG) emissions, crop yield, and soil or plant health. The special issue is divided into 4 sections that include (i) Estimating Crop Residue Removal and Modeling; (ii) Cultural Practice Impact on Soil Health; (iii) Residue Removal Impact on Soil and Plant Health; and (iv) Cultural Practice Impact on Carbon Storage and Greenhouse Gas Emissions.

Abstract  Arbuscular mycorrhizal fungi alleviate drought stress in their host plants via the direct uptake and transfer of water and nutrients through the fungal hyphae to the host plants. To quantify the contribution of the hyphae to plant water uptake, a new split-root hyphae system was designed and employed on barley grown in loamy soil inoculated with Glomus intraradices under well-watered and drought conditions in a growth chamber with a 14-h light period and a constant temperature (15 °C; day/night). Drought conditions were initiated 21 days after sowing, with a total of eight 7-day drying cycles applied. Leaf water relations, net photosynthesis rates, and stomatal conductance were measured at the end of each drying cycle. Plants were harvested 90 days after sowing. Compared to the control treatment, the leaf elongation rate and the dry weight of the shoots and roots were reduced in all plants under drought conditions. However, drought resistance was comparatively increased in the mycorrhizal host plants, which suffered smaller decreases in leaf elongation, net photosynthetic rate, stomatal conductance, and turgor pressure compared to the non-mycorrhizal plants. Quantification of the contribution of the arbuscular mycorrhizal hyphae to root water uptake showed that, compared to the non-mycorrhizal treatment, 4 % of water in the hyphal compartment was transferred to the root compartment through the arbuscular mycorrhizal hyphae under drought conditions. This indicates that there is indeed transport of water by the arbuscular mycorrhizal hyphae under drought conditions. Although only a small amount of water transport from the hyphal compartment was detected, the much higher hyphal density found in the root compartment than in the hyphal compartment suggests that a larger amount of water uptake by the arbuscular mycorrhizal hyphae may occur in the root compartment.

Abstract  Winter cover crops (WCCs) provide agronomic and environmental benefits, although their impacts on subsequent crop yields have been reported to vary across regions, soils, or under different farm practices. To address the variability in response, previous qualitative and quantitative reviews have summarized the overall yield effects of WCCs. However, the results from such reviews need constant revision as new research is published and interest in the conservation benefits of WCCs increases. Here, we update a previous meta-analysis of WCC effects on corn (Zea mays) yields, which summarized peer-reviewed research from the United Sates and Canada that was published between 1965 and 2004. Our updated data set (1965 to 2015) comprises 268 observations from 65 studies conducted in different regions of the United States and Canada, and includes information about the management practices utilized (i.e., WCC species, nitrogen [N] fertilization, termination date, tillage, etc.). The effect-size was the response ratio (RR), defined as corn yield following WCCs relative to yield after no cover crop (NC). As in the previous meta-analysis, our results showed a neutral to positive contribution of WCCs to corn yields. On average, grass WCCs neither increased nor decreased corn yields, although corn grown for grain yielded relatively higher than silage corn after grass WCCs. Legume WCCs resulted in subsequent higher corn yields by 30% to 33% when N fertilizer rates were low or the tillage system shifted from conventional tillage (CT) to no-tillage (NT). Mixture WCCs increased corn yields by 30% when the cover crop was late terminated (zero to six days before subsequent corn). Evidence of 65 years of research showed that uncertainty around the RR has decreased and corn yield response to WCCs has stabilized over time. Our results suggest that benefits of WCCs do not result in reduced corn productivity if properly managed.

Abstract  Soil is one of the most important natural resources and medium for plant growth. Anthropogenic interventions such as tillage, irrigation, and fertilizer application can affect the health of the soil. Use of fertilizer nitrogen (N) for crop production influences soil health primarily through changes in organic matter content, microbial life, and acidity in the soil. Soil organic matter (SOM) constitutes the storehouse of soil N. Studies with N-15-labelled fertilizers show that in a cropping season, plants take more N from the soil than from the fertilizer. A large number of long-term field experiments prove that optimum fertilizer N application to crops neither resulted in loss of organic matter nor adversely affected microbial activity in the soil. Fertilizer N, when applied at or below the level at which maximum yields are achieved, resulted in the build-up of SOM and microbial biomass by promoting plant growth and increasing the amount of litter and root biomass added to soil. Only when fertilizer N was applied at rates more than the optimum, increased residual inorganic N accelerated the loss of SOM through its mineralization. Soil microbial life was also adversely affected at very high fertilizers rates. Optimum fertilizer use on agricultural crops reduces soil erosion but repeated application of high fertilizer N doses may lead to soil acidity, a negative soil health trait. Site-specific management strategies based on principles of synchronization of N demand by crops with N supply from all sources including soil and fertilizer could ensure high yields, along with maintenance of soil health. Balanced application of different nutrients and integrated nutrient management based on organic manures and mineral fertilizers also contributed to soil health maintenance and improvement. Thus, fertilizer N, when applied as per the need of the field crops in a balanced proportion with other nutrients and along with organic manures, if available with the farmer, maintains or improves soil health rather than being deleterious.

Abstract  Soil microorganisms (bacteria, fungi) and microfauna (nematodes, protozoa) have been shown to be sensitive to organic amendments, but few experiments have investigated the responses of all these organisms simultaneously and across the soil profile. We investigated the impact of organic amendment and tillage on the soil food web at two depths in a field experiment. Over three growing seasons, field plots received seasonal organic amendment that was either incorporated into the soil (tilled) or not (no-till) as part of a tomato/soybean/corn cropping system. Un-amended, control plots that were either tilled or no-till were also included. We hypothesized that the addition of amendments would have a bottom–up effect on the soil food web, positively influencing the abundance of microorganisms, protozoa, and nematodes, primarily in the surface layers of the soil, but that this effect could be extended into deeper layers via tillage. Organic amendment had positive effects on most measured variables, including organic matter, respiration, protozoan and nematode density, and the abundance of PLFA biomarkers for bacteria and fungi. These effects were more pronounced in the 0–5 cm depth, but most variables increased with amendment in the deeper layer as well, especially with tillage. Denaturing Gradient Gel Electrophoresis (DGGE) of bacterial rDNA fragments indicated that distinct bacterial communities were selected for among tillage and amendment treatments and depths. Nematode faunal indices were not influenced by amendment, however. Increased nematode density in amended soils encompassed all trophic groups of free-living nematodes, with the greatest response among fungal-feeders, particularly with tillage. Increased biomass of microorganisms and decomposer microfauna in amended, tilled soils (0–5 cm depth) corresponded with a decline in the abundance of plant-parasitic nematodes. In control soils (0–5 cm depth), tillage reduced the relative abundance of fungal-feeding nematodes and increased the density of bacterial-feeding nematodes, in particular nematode species contributing to the Enrichment Index. When combined with organic amendment however, tillage was associated with increases in fungal-feeding nematodes and fungal biomarker PLFA. The results of this study suggest that when combined with amendment, tillage enhances the soil food web beyond the effect of amendment alone and is associated with declines in plant-parasitic nematodes.

Abstract  Switchgrass (Panicum virgatum L.) and big bluestem (Andropogon gerdardii Vitman) are potential bioenergy feedstocks. Perennial grasses managed as bioenergy feedstock require nitrogenous inputs, which can cause N2O emission and, thereby, alter their effectiveness to mitigate greenhouse gas (GHG) emission. Few studies have measured N2O emission from perennial grasses managed as feedstock. The objectives of this study were to compare N2O flux and soil organic C (SOC) storage between (i) grasses with legume companion crops or with nitrogenous fertilizer, (ii) two grass harvest times (autumn and spring), and (iii) perennial systems and corn (Zea maize L.)-soybean [Glycine max (L.) Merr.] (C-S) rotation, all without tillage. Nitrous oxide flux was measured from May 2009 to May 2012, and SOC was measured in 2000, 2006, and 2011. Big bluestem-clover (Dalea) and switchgrass-clover treatments had dramatically reduced annual N2O emissions and yield-scaled emissions compared with the respective grasses with urea fertilizer. Cumulative N2O emission was 14 to 40% greater in the big bluestem-spring and switchgrass-spring treatments compared with respective autumn-harvested treatments. The average cumulative emission in fertilized big bluestem and switchgrass treatments (18.5 kg N2O-N ha(-1)) exceeded that of the C-S rotation (12.7 kg N2O-N ha(-1)). The emission factor (EF) for fertilized grasses averaged 2.5%, corn averaged 1.05%, and C-S rotation averaged 1.9%. The SOC storage by perennial grasses was limited to 0 to 5 cm and thus may not be adequate to offset N2O emission. Nitrogen management refinement is recommended for herbaceous perennials to optimize biomass production and minimize N2O emission.

Abstract  Row crop production on highly erodible soils of the Piedmont in the southeastern USA is often limited by surface sealing, excessive surface water runoff, soil erosion, and low crop yields. The effects of four tillage practices on corn (Zea mays L.) growth and soil properties on two crust-prone soils were evaluated. Tillage treatments at two Piedmont locations, Oxford and Reidsville, NC, were no-till (NT), in-row subsoiling (IRS) (1987 only), chisel plow (CP), and moldboard plow-disk (MP). Residue cover was 1% for MP and ranged from 75 to 87% for NT and 38 to 27% for CP. The interaction between tillage, soil properties, and plant performance was complex. Mean bulk density of the Ap horizon at Reidsville for the 2-yr period was 1.56 Mg m(-3) for NT, compared with 1.48 Mg m(-3) for CP and 1.46 Mg m(-3) for MP. Cone index was not affected by tillage but was greatest in the trafficked interrow, 3.50 MPa, compared with 1.91 and 1.09 MPa in the row and nontrafficked interrow, respectively. Mean corn grain yield for the four year-locations was least, 1.23 Mg ha(-1), for MP, compared with 2.97 Mg ha(-1) for NT and 2.44 Mg ha(-1) for CP; mean yield for IRS in 1987 was 3.69 Mg ha(-1). Tillage practices leaving crop residues on the soil surface, such as NT, CP, and IRS, can reduce or eliminate surface crusting, increase infiltration, and reduce surface runoff and soil loss while increasing crop yield.

Abstract  Cellulosic crops are projected to provide a large fraction of transportation energy needs by mid-century. However, the anticipated land requirements are substantial, which creates a potential for environmental harm if trade-offs are not sufficiently well understood to create appropriately prescriptive policy. Recent empirical findings show that cellulosic bioenergy concerns related to climate mitigation, biodiversity, reactive nitrogen loss, and crop water use can be addressed with appropriate crop, placement, and management choices. In particular, growing native perennial species on marginal lands not currently farmed provides substantial potential for climate mitigation and other benefits.

Abstract  Producing biofuel feedstocks on current agricultural land raises questions of a food-vs.-fuel' trade-off. The use of current or former Conservation Reserve Program (CRP) land offers an alternative; yet the volumes of ethanol that could be produced and the potential environmental impacts of such a policy are unclear. Here, we applied the Environmental Policy Integrated Climate model to a US Department of Agriculture database of over 200000 CRP polygons in Iowa, USA, as a case study. We simulated yields and environmental impacts of growing three cellulosic biofuel feedstocks on CRP land: (i) an Alamo-variety switchgrass (Panicum virgatum L.); (ii) a generalized mixture of C4 and C3 grasses; (iii) and no-till corn (Zea mays L.) with residue removal. We simulated yields, soil erosion, and soil carbon (C) and nitrogen (N) stocks and fluxes. We found that although no-till corn with residue removal produced approximately 2.6-4.4 times more ethanol per area compared to switchgrass and the grass mixture, it also led to 3.9-4.5 times more erosion, 4.4-5.2 times more cumulative N loss, and a 10% reduction in total soil carbon as opposed to a 6-11% increase. Switchgrass resulted in the best environmental outcomes even when expressed on a per liter ethanol basis. Our results suggest planting no-till corn with residue removal should only be done on low slope soils to minimize environmental concerns. Overall, this analysis provides additional information to policy makers on the potential outcome and effects of producing biofuel feedstocks on current or former conservation lands.

Abstract  A socioeconomic model is used to estimate the land-use implications on the U.S. Conservation Reserve Program from potential increases in second-generation biofuel production. A baseline scenario with no second-generation biofuel production is compared to a scenario where the Renewable Fuels Standard (RFS2) volumes are met by 2022. We allow for the possibility of converting expiring CRP lands to alternative uses such as conventional crops, dedicated second-generation biofuel crops, or harvesting existing CRP grasses for biomass. Results indicate that RFS2 volumes (RFS2-v) can be met primarily with crop residues (78% of feedstock demand) and woody residues (19% of feedstock demand) compared with dedicated biomass (3% of feedstock demand), with only minimal conversion of cropland (0.27 million hectares, <1% of total cropland), pastureland (0.28 million hectares of pastureland, <1% of total pastureland), and CRP lands (0.29 million hectares of CRP lands, 3% of existing CRP lands) to biomass production. Meeting RFS2 volumes would reduce CRP re-enrollment by 0.19 million hectares, or 4%, below the baseline scenario where RFS2 is not met. Yet under RFS2-v scenario, expiring CRP lands are more likely to be converted to or maintain perennial cover, with 1.78 million hectares of CRP lands converting to hay production, and 0.29 million hectares being harvested for existing grasses. A small amount of CRP is harvested for existing biomass, but no conversion of CRP to dedicated biomass crops, such as switchgrass, are projected to occur. Although less land is enrolled in CRP under RFS2-v scenario, total land in perennial cover increases by 0.15 million hectares, or 2%, under RFS2-v. Sensitivity to yield, payment and residue retention assumptions are evaluated.

Abstract  Algae are a group of ubiquitous photosynthetic organisms comprising eukaryotic green algae and Gram-negative prokaryotic cyanobacteria, which have immense potential as a bioresource for various industries related to biofuels, pharmaceuticals, nutraceuticals and feed. This fascinating group of organisms also has applications in modern agriculture through facilitating increased nutrient availability, maintaining the organic carbon and fertility of soil, and enhancing plant growth and crop yields, as a result of stimulation of soil microbial activity. Several cyanobacteria provide nitrogen fertilization through biological nitrogen fixation and through enzymatic activities related to interconversions and mobilization of different forms of nitrogen. Both green algae and cyanobacteria are involved in the production of metabolites such as growth hormones, polysaccharides, antimicrobial compounds, etc., which play an important role in the colonization of plants and proliferation of microbial and eukaryotic communities in soil. Currently, the development of consortia of cyanobacteria with bacteria or fungi or microalgae or their biofilms has widened their scope of utilization. Development of integrated wastewater treatment and biomass production systems is an emerging technology, which exploits the nutrient sequestering potential of microalgae and its valorisation. This review focuses on prospects and challenges of application of microalgae in various areas of agriculture, including crop production, protection and natural resource management. An overview of the recent advances, novel technologies developed, their commercialization status and future directions are also included.

Abstract  Soil and foliar arthropod populations in agricultural settings respond to environmental disturbance and degradation, impacting functional biodiversity in agroecosystems. The objective of this study was to evaluate system level management effects on soil and foliar arthropod abundance and diversity in corn and soybean. Our field experiment was a completely randomized block design with three replicates for five farming systems which included: Conventional clean till, conventional long rotation, conventional no-till, organic clean till, and organic reduced till. Soil arthropod sampling was accomplished by pitfall trapping. Foliar arthropod sampling was accomplished by scouting corn and sweep netting soybean. Overall soil arthropod abundance was significantly impacted by cropping in corn and for foliar arthropods in soybeans. Conventional long rotation and organic clean till systems were highest in overall soil arthropod abundance for corn while organic reduced till systems exceeded all other systems for overall foliar arthropod abundance in soybeans. Foliar arthropod abundance over sampling weeks was significantly impacted by cropping system and is suspected to be the result of in-field weed and cover crop cultivation practices. This suggests that the sum of management practices within production systems impact soil and foliar arthropod abundance and diversity and that the effects of these systems are dynamic over the cropping season. Changes in diversity may be explained by weed management practices as sources of disturbance and reduced arthropod refuges via weed reduction. Furthermore, our results suggest agricultural systems lower in management intensity, whether due to organic practices or reduced levels of disturbance, foster greater arthropod diversity.

Abstract  Algae are being intensively researched as a potential bioenergy feedstock. Although algae are more productive per area of cultivation compared to first-generation biofuel feedstocks, its production may not be economically sustainable without high-value coproducts. One of many possible coproducts is algal residue following lipid extraction that might be used as a soil amendment for agricultural production. This experiment was aimed at determining, under laboratory conditions, the effects of lipid-extracted algae (LEA) (Nannochloropsis salina) amendment on soil C and N mineralization, soil microbial biomass, and soil pH and salinity over time. Soil organic C measured 392-d after amending soil with 1.5% or 3.0% LEA (dry weight basis) was increased by approximately 0.2% and 03% organic C (OC), respectively, compared to the control. Approximately 50% of added LEA-C was mineralized compared with 65% of added wheat (Triticum aestivum L.) straw-C. Lipid-extracted algae application may be one means of increasing OC: however, problems with excess soil salinity, sodicity, and nitrate-N may occur at high (3.0% or greater) addition rates.

Abstract  Soil tillage practices affect the soil microbial community in various ways, with possible consequences for nitrogen (N) losses, plant growth and soil organic carbon (C) sequestration. As microbes affect soil organic matter (SOM) dynamics largely through their activity, their impact may not be deduced from biomass measurements alone. Moreover, residual microbial tissue is thought to facilitate SOM stabilization, and to provide a long term integrated measure of effects on the microorganisms. In this study, we therefore compared the effect of reduced (RT) and conventional tillage (CT) on the biomass, growth rate and residues of the major microbial decomposer groups fungi and bacteria. Soil samples were collected at two depths (0–5 cm and 5–20 cm) from plots in an Irish winter wheat field that were exposed to either conventional or shallow non-inversion tillage for 7 growing seasons. Total soil fungal and bacterial biomasses were estimated using epifluorescence microscopy. To separate between biomass of saprophytic fungi and arbuscular mycorrhizae, samples were analyzed for ergosterol and phospholipid fatty acid (PLFA) biomarkers. Growth rates of saprophytic fungi were determined by [14C]acetate-in-ergosterol incorporation, whereas bacterial growth rates were determined by the incorporation of 3H-leucine in bacterial proteins. Finally, soil contents of fungal and bacterial residues were estimated by quantifying microbial derived amino sugars. Reduced tillage increased the total biomass of both bacteria and fungi in the 0–5 cm soil layer to a similar extent. Both ergosterol and PLFA analyses indicated that RT increased biomass of saprophytic fungi in the 0–5 cm soil layer. In contrast, RT increased the biomass of arbuscular mycorrhizae as well as its contribution to the total fungal biomass across the whole plough layer. Growth rates of both saprotrophic fungi and bacteria on the other hand were not affected by soil tillage, possibly indicating a decreased turnover rate of soil microbial biomass under RT. Moreover, RT did not affect the proportion of microbial residues that were derived from fungi. In summary, our results suggest that RT can promote soil C storage without increasing the role of saprophytic fungi in SOM dynamics relative to that of bacteria.

Abstract  To develop a more sustainable bio-based economy, an increasing amount of carbon for industrial applications and biofuel will be obtained from bioenergy crops. This may result in intensified land use and potential conflicts with other ecosystem services provided by soil, such as control of greenhouse gas emissions, carbon sequestration, and nutrient dynamics. A growing number of studies examine how bioenergy crops influence carbon and nitrogen cycling. Few studies, however, have combined such assessments with analysing both the immediate effects on the provisioning of soil ecosystem services as well as the legacy effects for subsequent crops in the rotation. Here, we present results from field and laboratory experiments on effects of a standard first-generation bioenergy crop (maize) and three different second-generation bioenergy crops (willow short rotation coppice (SRC), Miscanthus x giganteus, switchgrass) on key soil quality parameters: soil structure, organic matter, biodiversity and growth and disease susceptibility of a major follow-up crop, wheat (Triticum aestivum). We analysed a 6-year field experiment and show that willow SRC, Miscanthus, and maize maintained a high yield over this period. Soil quality parameters and legacy effects of Miscanthus and switchgrass were similar or performed worse than maize. In contrast, willow SRC enhanced soil organic carbon concentration (0-5cm), soil fertility, and soil biodiversity in the upper soil layer when compared to maize. In a greenhouse experiment, wheat grown in willow soil had higher biomass production than when grown in maize or Miscanthus soil and exhibited no growth reduction in response to introduction of a soil-borne (Rhizoctonia solani) or a leaf pathogen (Mycosphaerella graminicola). We conclude that the choice of bioenergy crops can greatly influence provisioning of soil ecosystem services and legacy effects in soil. Our results imply that bioenergy crops with specific traits might even enhance ecosystem properties through positive legacy effects.

Abstract  Corn (Zea mays L.) stover is a global resource used for livestock, fuel, and bioenergy feedstock, but excessive stover removal can decrease soil organic C (SOC) stocks and deteriorate soil health. Many site-specific stover removal experiments report accrual rates and SOC stock effects, but a quantitative, global synthesis is needed to provide a scientific base for long-term energy policy decisions. We used 409 data points from 74 stover harvest experiments conducted around the world for a meta-analysis and meta-regression to quantify removal rate, tillage, soil texture, and soil sampling depth effects on SOC. Changes were quantified by: (a) comparing final SOC stock differences after at least 3 years with and without stover removal and (b) calculating SOC accrual rates for both treatments. Stover removal generally reduced final SOC stocks by 8% in the upper 0–15 or 0–30 cm, compared to stover retained, irrespective of soil properties and tillage practices. A more sensitive meta-regression analysis showed that retention increased SOC stocks within the 30–150 cm depth by another 5%. Compared to baseline values, stover retention increased average SOC stocks temporally at a rate of 0.41 Mg C ha−1 year−1 (statistically significant at p < 0.01 when averaged across all soil layers). Although SOC sequestration rates were lower with stover removal, with moderate (<50%) removal they can be positive, thus emphasizing the importance of site-specific management. Our results also showed that tillage effects on SOC stocks were inconsistent due to the high variability in practices used among the experimental sites. Finally, we conclude that research and technological efforts should continue to be given high priority because of the importance in providing science-based policy recommendations for long-term global carbon management.

Abstract  Best management practices that reduce potential phosphorus (P) loss and provide flexibility in P fertilizer management are needed to help producers protect water quality while maintaining crop yield. This study examined the impacts of P fertilizer management (no P, fall broadcast P, and spring injected P) and cover crop use on annual concentrations and loads of sediment, total P, and dissolved reactive P (DRP) in edge-of-field runoff from a no-till corn (Zea mays)-soybean (Glycine max) rotation in the Central Great Plains, USA, from September 2015 through September 2019. The spring injected P fertilizer treatment generally had 19% less total P and 33% less DRP loss compared to the fall broadcast treatment, confirming the importance of P fertilizer management as a practice for reducing P loss. The addition of a cover crop had an inconsistent effect on total P loss, with no effect in 2016 and 2017, increasing loss in 2018 by 56%, and decreasing it in 2019 by 40%. The inconsistent impact of cover crops on total P loss was related to cover crop effects on sediment loss. Although cover crop impacts on total P losses were inconsistent, the addition of a cover crop increased DRP loss in three of four years. Cover crop use consistently reduced sediment loss, with greater sediment reduction when P fertilizer was applied. Results from this study highlight the benefit of cover crops for reducing sediment loss and the continued need for proper fertilizer management to reduce P loss from agricultural fields.

Abstract  Long-term loss of soil C stocks under conventional tillage and accrual of soil C following adoption of no-tillage have been well documented. No-tillage use is spreading, but it is common to occasionally till within a no-till regime or to regularly alternate between till and no-till practices within a rotation of different crops. Short-term studies indicate that substantial amounts of C can be lost from the soil immediately following a tillage event, but there are few field studies that have investigated the impact of infrequent tillage on soil C stocks. How much of the C sequestered under no-tillage is likely to be lost if the soil is tilled? What are the longer-term impacts of continued infrequent no-tillage? If producers are to be compensated for sequestering C in soil following adoption of conservation tillage practices, the impacts of infrequent tillage need to be quantified. A few studies have examined the short-term impacts of tillage on soil C and several have investigated the impacts of adoption of continuous no-tillage. We present: (1) results from a modeling study carried out to address these questions more broadly than the published literature allows, (2) a review of the literature examining the short-term impacts of tillage on soil C, (3) a review of published studies on the physical impacts of tillage and (4) a synthesis of these components to assess how infrequent tillage impacts soil C stocks and how changes in tillage frequency could impact soil C stocks and C sequestration. Results indicate that soil C declines significantly following even one tillage event (1–11% of soil C lost). Longer-term losses increase as frequency of tillage increases. Model analyses indicate that cultivating and ripping are less disruptive than moldboard plowing, and soil C for those treatments average just 6% less than continuous NT compared to 27% less for CT. Most (80%) of the soil C gains of NT can be realized with NT coupled with biannual cultivating or ripping.

Abstract  Field crop traits have and are experiencing significant changes due to genetic and agronomic improvements. How these changes affect regional water quantity and quality processes has not been clarified. The St. Joseph River Watershed (SJRW) located in the U.S. Corn Belt was selected as a case study area. Crop (corn and soybean) trait improvements in the past decades were reviewed and summarized and include changes of growing degree days (GDD), leaf area index (LAI), light utilization (LU), drought tolerance (DT), nutrient content (NC), and harvest index (HI). Based on a calibrated 9-year (from 2011 to 2019) SWAT (Soil and Water Assessment Tool) simulation in SJRW, sensitivities of the above crop traits to yield, ETa, stream flow, tile flow, surface runoff, and nutrient loads (NO3-N, TN, soluble-P, and TP) were analyzed. Crop traits and their corresponding SWAT parameters for the 2010s were obtained from model calibration and used as the baseline/current scenario; for the 1980s, they were summarized from literature review and used as an historical scenario, while those for the 2040s were determined by assuming crop traits are changing linearly with time and projected as the future scenario. Water quantity and quality changes under the historical and future crop scenarios were compared with the baseline/ current simulation. Results showed LU and DT were the most sensitive crop traits to water quantity (i.e., ETa, stream flow, tile flow, and surface runoff), while HI was the most sensitive to nutrient loads. The impacts of crop improvements on nutrient loads were more significant than on water budgets. Compared with the baseline, the historical and future scenarios resulted in 1.5 -2.0% changes of stream flow, 6.8 -18.6% changes of nitrogen loads (NO3-N and TN) and 2.6 -3.9% changes of phosphorus loads (soluble-P, and TP) in the stream flow, annually. Moreover, in certain months, these changes can reach about 12% for stream flow, 42% for nitrogen loads, and 12% for phosphorus loads. Nitrogen losses by tile drainage and percolation, and phosphorus losses by surface runoff and tile drainage were most significantly affected by the crop improvements. Future work should consider expected crop improvements when studying long-term hydrology and nutrient cycles in agricultural watersheds.

Abstract  We examined how chronic nitrogen (N) enrichment of pine and hardwood forest stands has affected the relative abundance, functional capacity, and activity of soil bacteria and fungi. During Fall 2002 we collected one soil core (5.6 cm diameter; organic horizon plus 10 cm of mineral soil) from each of four 5 mÎ5 m subplots within the control, low N (5 g N m-2 per year), and high N (15 g N m-2 per year) plots in both the hardwood and pine stands at the Chronic Nitrogen Amendment Study at Harvard Forest. The samples were analyzed for total and active bacterial and fungal biomass, microbial catabolic response profiles, the activities of cellulolytic and ligninolytic enzymes, and total, labile and microbially derived organic carbon (C). Live, fine roots were also collected from the control and low N pine plots and analyzed for ectomycorrhizal fungal community composition and diversity. Active fungal biomass was 27û61% and 42û69% lower in the fertilized compared to control plots in the hardwood and pine stands, respectively. Active bacterial biomass was not greatly affected by N additions, resulting in significantly lower fungal:bacterial biomass ratios in the N-treated plots. This shift in microbial community composition was accompanied by a significant reduction in the activity of phenol oxidase, a lignin-degrading enzyme produced by white-rot fungi. In the pine stand, ectomycorrhizal fungal community diversity was lower in the low N-treated plot than in the control plot. Differences in ectomycorrhizal community structure were also detected between control and fertilized pine plots, including a reduction in those species with the highest relative frequencies in the control community. Finally, N enrichment altered the pattern of microbial substrate use, with the relative response to the addition of carboxylic acids and carbohydrates being significantly lower in the N-treated plots, even after the data were normalized to account for differences in microbial biomass. These patterns are consistent with lower decomposition rates and altered N cycling observed previously at this site.

Abstract  The growth in ethanol production in the United States has sparked interest in potential land-use change and the associated environmental impacts that may occur in order to accommodate the increasing demand for grain feedstocks. In this study water quality and sustainability indicators are used to evaluate the impacts of land-use change to increase corn and grain sorghum acreage for biofuel production in the Perry Lake watershed in northeast Kansas. Water quality indicators include sediment loads per converted land acreage and the relative increase of total nitrogen, total phosphorus and sediment loads compared to the baseline conditions. Sustainability indicators include land-use, water use, and nutrient use efficiencies. Hay, Conservation Reserve Program (CRP), and winter wheat were selected as targeted land-uses for conversion to biofuel feedstocks. The Soil and Water Assessment Tool (SWAT) was used to evaluate 6 different scenarios, each at 10 land-use change increments, for a total of 60 simulations. Results demonstrate that increased corn production generates significantly greater sediment loads than increased grain sorghum production and larger relative increases in nutrient loads. Expansion of corn or grain sorghum cropland by replacing hay or CRP land-uses resulted in the highest sediment loads and relative increases in nutrient loads. Expansion of corn or grain sorghum by replacing winter wheat cropland produced the lowest relative changes in nutrient and sediment loads and therefore may be a more sustainable land-use change. Corn had a higher yield potential per km(2) compared to grain sorghum, resulting in better land, nutrient and water use efficiencies.

Abstract  Ecological intensification of agriculture (El) aims to conserve and promote biodiversity and the sustainable use of associated ecosystem services to support resource-efficient production. In many cases El requires fundamental changes in farm and landscape management as well as the organizations and institutions that support agriculture. Ecologists can facilitate El by engaging with stakeholders and, in the process, by generating "actionable knowledge" (that is, knowledge that specifically supports stakeholder decision making and consequent actions). Using three case studies as examples, we propose four principles whereby science can improve the delivery of actionable knowledge for EI: (1) biodiversity conservation helps to ensure the delivery of ecosystem services, (2) management of ecosystem services benefits from a landscape-scale approach, (3) ecosystem service trade-offs and synergies need to be articulated, and (4) EI is associated with complex social dynamics involving farmers, governments, researchers, and related institutions. These principles have the potential to enhance adoption of EI, but institutional and policy challenges remain.